Publication: Morphological, Phylogenetic and Toxinological Characterization of Potentially Harmful Algal Species from the Marine Coastal Waters of Buenos Aires Province (Argentina)
Date
2023
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Abstract
In the framework of a monitoring program of harmful microalgae from the marine coastal waters of the Buenos Aires Province, seven strains were isolated and characterized by morphological and molecular analysis (LSU rDNA partial sequencing, D1–D3 regions). Established strains belonged to Alexandrium catenella, Protoceratium reticulatum and Pseudo-nitzschia multiseries. The toxinological profile of the target strains were determined by UHPLC-FLD equipment for paralytic shellfish toxins (PSTs) and LC-MS/MS for lipophilic (LSTs) and amnesic toxins (ASTs). The toxin profile varied in the four strains of A. catenella, the predominant compounds were gonyautoxins (GTXs) GTX2,3 and GTX1,4 for strains LPCc001 and LPCc004, and N-sulfocarbamoyl toxins (Cs) C1,2 and GTX1,4 for strains LPCc002 and LPCc008. The obtained cellular toxicity values were moderate-to-high (12.38–46.40 pg saxitoxin equiv. cell−1). The toxin profile of P. reticulatum was dominated by yessotoxins (YTXs) (up to 94.40 pg cell−1) accompanied by homo-yessotoxin (Homo-YTX) traces. In P. multiseries, the toxin profiles were dominated by domoic acid (DA) (1.62 pg cell−1 and 1.09 pg cell−1) and secondarily by Isomer A (Iso-A), Epi-domoic acid (Epi-DA), Isomer-E (Iso-E) and Isomer-D (Iso-D). This study provides detailed information about representative HAB species in the area, useful for resource management, risk evaluation and related research on toxic dinoflagellates and diatoms.
Description
Fil: Tardivo Kubis, Jonas A. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina.
Fil: Tardivo Kubis, Jonas A. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.
Fil: Rodríguez Hernández, Francisco. Instituto Español de Oceanografía (IEO); España.
Fil: Rossignoli, Araceli. Instituto Español de Oceanografía (IEO); Argentina.
Fil: Riobó, Pilar. Consejo Superior de Investigaciones Científicas (CSIC); España.
Fil: Sar, Eugenia Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. Fil: Sar, Eugenia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.
Fil: Sunesen, Inés. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. Fil: Sunesen, Inés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.
Fil: Rodríguez Hernández, Francisco. Instituto Español de Oceanografía (IEO); España.
Fil: Rossignoli, Araceli. Instituto Español de Oceanografía (IEO); Argentina.
Fil: Riobó, Pilar. Consejo Superior de Investigaciones Científicas (CSIC); España.
Fil: Sar, Eugenia Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. Fil: Sar, Eugenia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.
Fil: Sunesen, Inés. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. Fil: Sunesen, Inés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.
Keywords
Alexandrium catenella, Molecular analysis, Morphology, Protoceratium reticulatum, Pseudo-nitzschia multiseries, Toxinological profiles