Person: Tardivo Kubis, Jonas A.
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Tardivo Kubis
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Jonas A.
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Publication A re-investigation of three Frenguelli's Caloneis taxa (Pinnulariaceae, Bacillariophyta) from Argentina(2017) Sunesen, Inés; Tardivo Kubis, Jonas A.; Sar, Eugenia AliciaPublication Morphological, Phylogenetic and Toxinological Characterization of Potentially Harmful Algal Species from the Marine Coastal Waters of Buenos Aires Province (Argentina)(2023) Tardivo Kubis, Jonas A.; RodrÃguez Hernández, Francisco; Rossignoli, Araceli; Riobó, Pilar; Sar, Eugenia Alicia; Sunesen, InésIn the framework of a monitoring program of harmful microalgae from the marine coastal waters of the Buenos Aires Province, seven strains were isolated and characterized by morphological and molecular analysis (LSU rDNA partial sequencing, D1–D3 regions). Established strains belonged to Alexandrium catenella, Protoceratium reticulatum and Pseudo-nitzschia multiseries. The toxinological profile of the target strains were determined by UHPLC-FLD equipment for paralytic shellfish toxins (PSTs) and LC-MS/MS for lipophilic (LSTs) and amnesic toxins (ASTs). The toxin profile varied in the four strains of A. catenella, the predominant compounds were gonyautoxins (GTXs) GTX2,3 and GTX1,4 for strains LPCc001 and LPCc004, and N-sulfocarbamoyl toxins (Cs) C1,2 and GTX1,4 for strains LPCc002 and LPCc008. The obtained cellular toxicity values were moderate-to-high (12.38–46.40 pg saxitoxin equiv. cell−1). The toxin profile of P. reticulatum was dominated by yessotoxins (YTXs) (up to 94.40 pg cell−1) accompanied by homo-yessotoxin (Homo-YTX) traces. In P. multiseries, the toxin profiles were dominated by domoic acid (DA) (1.62 pg cell−1 and 1.09 pg cell−1) and secondarily by Isomer A (Iso-A), Epi-domoic acid (Epi-DA), Isomer-E (Iso-E) and Isomer-D (Iso-D). This study provides detailed information about representative HAB species in the area, useful for resource management, risk evaluation and related research on toxic dinoflagellates and diatoms.Publication Morphology, genetics and toxin profile of Prorocentrum texanum (Dinophyceae) from Argentinian marine coastal waters(2020) Sunesen, Inés; RodrÃguez Hernández, Francisco; Aguiar Juárez, Delfina; Tardivo Kubis, Jonas A.; Lavigne, Andrea S.; Rossignoli, Araceli; Riobó, Pilar; Sar, Eugenia AliciaTwo strains of Prorocentrum texanum (LPCc020 and LPCc032) were established from coastal waters of the Province of Buenos Aires, Argentina. Field and cultured materials were examined using light and scanning electron microscopy, and the planktonic species Prorocentrum texanum, a known okadaic acid producer, was identified. We compared morphology, genetics and toxin profile of P. texanum based on material from Argentina in relation to strains from the Gulf of México. Our specimens and those described in the protologue of P. texanum var. texanum had similar shape, thecal ornamentation distribution patterns of large and small pores, and morphology of wings supported by platelets 1 and 4, respectively. Morphometric differences occurred in the ranges of length, depth and diameter of thecal plate pores. Morphology of the periflagellar area was described from Argentinean strains. SSU, ITS and LSU rDNA-based phylogenies of strains LPCc020 and LPCc032 placed them with other sequences of P. texanum. ITS rDNA showed that the most closely species, in order, are P. micans, P. steidingerae, P. mexicanum/P. rhathymum, and P. koreanum. The ability of the Argentinian strains to produce marine biotoxins was assessed, but, no toxins were detected. This is the first report of P. texanum outside the Gulf of México.Publication Morphological and molecular characterization of Heterocapsa claromecoensis sp. nov. (Peridiniales, Dinophyceae) from Buenos Aires coastal waters (Argentina)(2020) Sunesen, Inés; RodrÃguez Hernández, Francisco; Tardivo Kubis, Jonas A.; Aguiar Juárez, Delfina; Risso, Antonella; Lavigne, Andrea S.; Tillmann, Urban; Sar, Eugenia Alicia; Wietkamp, StephanA new species of the marine dinophyte genus Heterocapsa Stein is described. Two clonal strains originating from Argentinean coastal waters were examined with light and electron microscopy and LSU and ITS rDNA sequence data were obtained. Heterocapsa claromecoensis sp. nov. is described as a distinctive species with a conical epitheca, a similar sized and rounded hypotheca, a single reticulate chloroplast situated in the periphery of the cell, and a large pyrenoid in the episome, above an ellipsoidal to rounded nucleus in the hyposome. The plate tabulation pattern of Po (pore plate), cp (cover plate), X (X-plate or canal plate), 5ꞌ, 3a, 7ꞌꞌ, 6c, 5s, 5ꞌꞌꞌ, 2ꞌꞌꞌꞌ was as for most other species of Heterocapsa. H. claromecoensis sp. nov. differs from all other species of Heterocapsa by the microarchitecture of two different types of organic body scales, which uniquely were different in height. Phylogenetic trees based on LSU and ITS rDNA sequences placed H. claromecoensis in a separate branch, with a sequence assigned to H. orientalis being the closest match based on LSU rDNA.Publication First record of the genus Togula (Gymnodiniales, Dinophycea) and of the species T. Jolla for the south-west atlantic coastal warters(2024) Sunesen, Inés; RodrÃguez Hernández, Francisco; Aguiar Juárez, Delfina; Tardivo Kubis, Jonas A.; Sar, Eugenia AliciaEn el marco de un programa de monitoreo de fitoplancton y biotoxinas implementado desde 2008 en aguas marinas costeras de la Provincia de Buenos Aires (Argentina), un dinoflagelado gimnodinioide, fotosintético, fue aislado de una muestra colectada en Santa Teresita, estableciéndose la cepa LPCc007. La cepa fue identificada como perteneciente al género Togula con microscopÃa óptica con base en los siguientes caracteres distintivos: morfologÃa de la célula dorso-ventralmente aplanada, y curso del cingulum, altamente asimétrico, descendente, con sus finales desplazados por cerca de uno a dos tercios de la longitud total de la célula y unidos por un surco intercingular oblicuo. El análisis morfológico comparativo entre las células vegetativas móviles de la cepa aislada y las tres especies del género descriptas hasta la fecha no reveló diferencias significativas que permitieran determinarla a nivel especÃfico. En cambio, el patrón de desarrollo de surcos longitudinales en el hipocono de células a punto de sufrir mitosis observado en la cepa estudiada, concuerda perfectamente con el descrito para T. jolla como un carácter diferencial. La identificación morfológica fue consistente con la filogenia basada en LSU ADNr (D1-D2) que mostró la agregación de la secuencia en el clado molecular correspondiente a T. jolla. Este es el primer reporte del género Togula y de la especie T. jolla para Argentina y aguas costeras del Sudoeste del Océano Atlántico.Publication Morphological, molecular and toxinological analyses of Alexandrium affine (Dinophyceae) from marine coastal waters of Buenos Aires Province (Argentina)(2023) Tardivo Kubis, Jonas A.; RodrÃguez Hernández, Francisco; Rossignoli, Araceli; Riobó, Pilar; Aguiar Juárez, Delfina; Sar, Eugenia Alicia; Sunesen, InésIn the framework of a monitoring program of harmful microalgae from the marine coastal waters of Buenos Aires Province to mitigate the impacts of harmful algal blooms on the aquaculture and marine life and to protect human health, a strain of Alexandrium affine (LPCc012) was isolated. Alexandrium affine is a worldwide distributed species reported as harmful algal bloom-forming, with toxin- and nontoxin-producing strains. Field and cultured materials from Buenos Aires Province were examined using light and scanning electron microscopy, morphologically and genetically (LSU rDNA partial sequencing) characterized, and compared with material from different regions worldwide. Toxin analyses using ultraperformance liquid chromatography with fluorescence detection and liquid chromatography-mass spectrometry methods failed to detect any paralytic or lipophilic shellfish toxins. This is the first record of A. affine from Argentina.Publication Primer reporte de Biecheleria brevisulcata (Suessiaceae, Dinophyceae) para Argentina(2023) Sunesen, Inés; RodrÃguez Hernández, Francisco; Tardivo Kubis, Jonas A.; Aguiar Juárez, Delfina; Sar, Eugenia AliciaEn el marco de un programa de monitoreo de fitoplancton y biotoxinas implementado desde 2008 en aguas marinas costeras de la Provincia de Buenos Aires (Argentina), ha sido aislada la cepa LPCc022 de un dinoflagelado woloszynskioide, nanoplanctónico, de paredes delgadas. La filogenia basada en LSU rDNA mostró que la cepa aislada pertenecÃa a un clado molecular correspondiente a Biecheleria brevisulcata. B. brevisulcata puede ser diferenciada mediante microscopÃa óptica de las especies B. baltica, B. halophila, B. cincta y B. tirezensis sobre la base de la ubicación del núcleo en la célula, el rango de tamaño de las células y los ambientes que habitan. Este es el primer reporte del género Biecheleria y la especie B. brevisulcata en Argentina y en general en aguas del Atlántico sudoccidental.Publication Monitoreo de microalgas nocivas y monitoreo de ficotoxinas en moluscos en la costa bonaerense(2018) Tardivo Kubis, Jonas A.; Lavigne, Andrea S.; Aguiar Juárez, Delfina; Risso, Antonella; Sar, Eugenia Alicia; Sunesen, InésNuestro grupo de trabajo ejecuta desde 2008 un monitoreo de microalgas y monitoreo de toxinas en moluscos de ambientes costeros bonaerenses, en conjunto con la Dirección Provincial de Pesca (DPP) del Ministerio de Agroindustria de la Provincia de Buenos Aires y el Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). El objetivo del proyecto es detectar tempranamente especies de microalgas nocivas toxÃgenas o no toxÃgenas capaces de producir efectos adversos para la salud humana, el medioambiente, la acuicultura y las actividades recreativas en zonas costeras y detectar toxinas en moluscos. Durante el perÃodo hemos determinado toxinas PSP asociadas a la presencia en el agua de las microalgas Alexandrium catenella y Gymnodinium catenatum y toxinas DSP asociadas a las microalgas Dinophysis acuminata y D. caudata. La información de estos eventos fue transferida a la DPP, encargada de imponer vedas a la extracción y consumo de moluscos en áreas costeras.